Construct: shRNA TRCN0000085758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172817.1-1752s1c1
DNA Barcode:
CCGCACTTAGAACATGCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp647 (239546)
Gene Description:
zinc finger protein 647
Transcript:
RefSeq NM_172817.1 (NON-CURRENT)
Match location:
Position 1752 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCACTTAGAACATGCACAA
Hairpin Sequence:
5'-CCGG-CCGCACTTAGAACATGCACAA-CTCGAG-TTGTGCATGTTCTAAGTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCACTTAGAACATGCACAACTCGAGTTGTGCATGTTCTAAGTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCACTTAGAACATGCACAACTCGAGTTGTGCATGTTCTAAGTGCGG-3'

Other clones with same target sequence:

(none)