Construct: shRNA TRCN0000085766

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177185.3-4020s1c1
DNA Barcode:
CCCAGAATTTACTAAAGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubn2 (320538)
Gene Description:
ubinuclein 2
Transcript:
RefSeq NM_177185.3 (NON-CURRENT)
Match location:
Position 4020 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085766)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAATTTACTAAAGAGTT
Hairpin Sequence:
5'-CCGG-CCCAGAATTTACTAAAGAGTT-CTCGAG-AACTCTTTAGTAAATTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAATTTACTAAAGAGTTCTCGAGAACTCTTTAGTAAATTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAATTTACTAAAGAGTTCTCGAGAACTCTTTAGTAAATTCTGGG-3'

Other clones with same target sequence:

(none)