Construct: shRNA TRCN0000085790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172598.2-2435s1c1
DNA Barcode:
GCCGTGCATTTAGCCATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdhd1 (218973)
Gene Description:
WD repeat and HMG-box DNA binding protein 1
Transcript:
RefSeq NM_172598.2 (NON-CURRENT)
Match location:
Position 2435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGCATTTAGCCATTAAA
Hairpin Sequence:
5'-CCGG-GCCGTGCATTTAGCCATTAAA-CTCGAG-TTTAATGGCTAAATGCACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGCATTTAGCCATTAAACTCGAGTTTAATGGCTAAATGCACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGCATTTAGCCATTAAACTCGAGTTTAATGGCTAAATGCACGGC-3'

Other clones with same target sequence:

(none)