Construct: shRNA TRCN0000085837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181404.3-826s1c1
DNA Barcode:
GTCGTCCATCAATTCCGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kank1 (107351)
Gene Description:
KN motif and ankyrin repeat domains 1
Transcript:
RefSeq NM_181404.3 (NON-CURRENT)
Match location:
Position 826 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGTCCATCAATTCCGTCAT
Hairpin Sequence:
5'-CCGG-GTCGTCCATCAATTCCGTCAT-CTCGAG-ATGACGGAATTGATGGACGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGTCCATCAATTCCGTCATCTCGAGATGACGGAATTGATGGACGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGTCCATCAATTCCGTCATCTCGAGATGACGGAATTGATGGACGAC-3'

Other clones with same target sequence:

(none)