Construct: shRNA TRCN0000085854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020589.1-1128s1c1
DNA Barcode:
CGAGAAACGTTTCCGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp467 (68910)
Gene Description:
zinc finger protein 467
Transcript:
RefSeq NM_020589.1 (NON-CURRENT)
Match location:
Position 1128 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAAACGTTTCCGCAAGAA
Hairpin Sequence:
5'-CCGG-CGAGAAACGTTTCCGCAAGAA-CTCGAG-TTCTTGCGGAAACGTTTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAAACGTTTCCGCAAGAACTCGAGTTCTTGCGGAAACGTTTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAAACGTTTCCGCAAGAACTCGAGTTCTTGCGGAAACGTTTCTCG-3'

Other clones with same target sequence:

(none)