Construct: shRNA TRCN0000085876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177239.1-73s1c1
DNA Barcode:
GAAATGATGAAAGTACAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mysm1 (320713)
Gene Description:
myb-like, SWIRM and MPN domains 1
Transcript:
RefSeq NM_177239.1 (NON-CURRENT)
Match location:
Position 73 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATGATGAAAGTACAGCAT
Hairpin Sequence:
5'-CCGG-GAAATGATGAAAGTACAGCAT-CTCGAG-ATGCTGTACTTTCATCATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATGATGAAAGTACAGCATCTCGAGATGCTGTACTTTCATCATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATGATGAAAGTACAGCATCTCGAGATGCTGTACTTTCATCATTTC-3'

Other clones with same target sequence:

(none)