Construct: shRNA TRCN0000085902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011565.2-409s1c1
DNA Barcode:
CTCGAAGGAAATCGAGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tead2 (21677)
Gene Description:
TEA domain family member 2
Transcript:
RefSeq NM_011565.2 (CURRENT)
Match location:
Position 409 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGAAGGAAATCGAGAGAAA
Hairpin Sequence:
5'-CCGG-CTCGAAGGAAATCGAGAGAAA-CTCGAG-TTTCTCTCGATTTCCTTCGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGAAGGAAATCGAGAGAAACTCGAGTTTCTCTCGATTTCCTTCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGAAGGAAATCGAGAGAAACTCGAGTTTCTCTCGATTTCCTTCGAG-3'

Other clones with same target sequence:

  1. TRCN0000301800