Construct: shRNA TRCN0000085942

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008186.2-632s1c1
DNA Barcode:
GCCAATCGTTTAAATGTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf2h1 (14884)
Gene Description:
general transcription factor II H, polypeptide 1
Transcript:
RefSeq NM_008186.2 (NON-CURRENT)
Match location:
Position 632 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085942)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATCGTTTAAATGTGAAT
Hairpin Sequence:
5'-CCGG-GCCAATCGTTTAAATGTGAAT-CTCGAG-ATTCACATTTAAACGATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATCGTTTAAATGTGAATCTCGAGATTCACATTTAAACGATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATCGTTTAAATGTGAATCTCGAGATTCACATTTAAACGATTGGC-3'

Other clones with same target sequence:

  1. TRCN0000288317