Construct: shRNA TRCN0000085974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007826.1-1742s1c1
DNA Barcode:
GCATCGACAAACTTTCTCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dach1 (13134)
Gene Description:
dachshund 1 (Drosophila)
Transcript:
RefSeq NM_007826.1 (NON-CURRENT)
Match location:
Position 1742 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000085974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCGACAAACTTTCTCTAA
Hairpin Sequence:
5'-CCGG-GCATCGACAAACTTTCTCTAA-CTCGAG-TTAGAGAAAGTTTGTCGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCGACAAACTTTCTCTAACTCGAGTTAGAGAAAGTTTGTCGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCGACAAACTTTCTCTAACTCGAGTTAGAGAAAGTTTGTCGATGC-3'

Other clones with same target sequence:

(none)