Construct: shRNA TRCN0000086005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017376.2-819s1c1
DNA Barcode:
GTATTGGACAAGACGTAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tef (21685)
Gene Description:
thyrotroph embryonic factor
Transcript:
RefSeq NM_017376.2 (NON-CURRENT)
Match location:
Position 819 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTGGACAAGACGTAAGAA
Hairpin Sequence:
5'-CCGG-GTATTGGACAAGACGTAAGAA-CTCGAG-TTCTTACGTCTTGTCCAATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTGGACAAGACGTAAGAACTCGAGTTCTTACGTCTTGTCCAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTGGACAAGACGTAAGAACTCGAGTTCTTACGTCTTGTCCAATAC-3'

Other clones with same target sequence:

  1. TRCN0000301986