Construct: shRNA TRCN0000086007

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017376.2-832s1c1
DNA Barcode:
CGTAAGAAGAACAATGTGGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tef (21685)
Gene Description:
thyrotroph embryonic factor
Transcript:
RefSeq NM_017376.2 (NON-CURRENT)
Match location:
Position 832 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086007)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAAGAAGAACAATGTGGCA
Hairpin Sequence:
5'-CCGG-CGTAAGAAGAACAATGTGGCA-CTCGAG-TGCCACATTGTTCTTCTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAAGAAGAACAATGTGGCACTCGAGTGCCACATTGTTCTTCTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAAGAAGAACAATGTGGCACTCGAGTGCCACATTGTTCTTCTTACG-3'

Other clones with same target sequence:

  1. TRCN0000331525