Construct: shRNA TRCN0000086061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178722.3-359s1c1
DNA Barcode:
CCTTCCTCAATTTCTGACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp438 (240186)
Gene Description:
zinc finger protein 438
Transcript:
RefSeq NM_178722.3 (NON-CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCTCAATTTCTGACCAA
Hairpin Sequence:
5'-CCGG-CCTTCCTCAATTTCTGACCAA-CTCGAG-TTGGTCAGAAATTGAGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCTCAATTTCTGACCAACTCGAGTTGGTCAGAAATTGAGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCTCAATTTCTGACCAACTCGAGTTGGTCAGAAATTGAGGAAGG-3'

Other clones with same target sequence:

(none)