Construct: shRNA TRCN0000086080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008636.2-1353s1c1
DNA Barcode:
GCAGGAAATTCGGCGTCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtf1 (17764)
Gene Description:
metal response element binding transcription factor 1
Transcript:
RefSeq NM_008636.2 (NON-CURRENT)
Match location:
Position 1353 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAAATTCGGCGTCTTTA
Hairpin Sequence:
5'-CCGG-GCAGGAAATTCGGCGTCTTTA-CTCGAG-TAAAGACGCCGAATTTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAAATTCGGCGTCTTTACTCGAGTAAAGACGCCGAATTTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAAATTCGGCGTCTTTACTCGAGTAAAGACGCCGAATTTCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000311783