Construct: shRNA TRCN0000086085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007855.1-256s1c1
DNA Barcode:
AGCAAGAAATCGAGCGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Twist2 (13345)
Gene Description:
twist basic helix-loop-helix transcription factor 2
Transcript:
RefSeq NM_007855.1 (NON-CURRENT)
Match location:
Position 256 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGAAATCGAGCGAAGAT
Hairpin Sequence:
5'-CCGG-AGCAAGAAATCGAGCGAAGAT-CTCGAG-ATCTTCGCTCGATTTCTTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGAAATCGAGCGAAGATCTCGAGATCTTCGCTCGATTTCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGAAATCGAGCGAAGATCTCGAGATCTTCGCTCGATTTCTTGCT-3'

Other clones with same target sequence:

(none)