Construct: shRNA TRCN0000086137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013827.1-751s1c1
DNA Barcode:
CCACCAAATGTGGCTTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtf2 (17765)
Gene Description:
metal response element binding transcription factor 2
Transcript:
RefSeq NM_013827.1 (NON-CURRENT)
Match location:
Position 751 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCAAATGTGGCTTTCAAA
Hairpin Sequence:
5'-CCGG-CCACCAAATGTGGCTTTCAAA-CTCGAG-TTTGAAAGCCACATTTGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCAAATGTGGCTTTCAAACTCGAGTTTGAAAGCCACATTTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCAAATGTGGCTTTCAAACTCGAGTTTGAAAGCCACATTTGGTGG-3'

Other clones with same target sequence:

(none)