Construct: shRNA TRCN0000086304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146251.2-2810s1c1
DNA Barcode:
GCAGCTAATCTTGCTGCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pnpla7 (241274)
Gene Description:
patatin-like phospholipase domain containing 7
Transcript:
RefSeq NM_146251.2 (NON-CURRENT)
Match location:
Position 2810 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTAATCTTGCTGCACAA
Hairpin Sequence:
5'-CCGG-GCAGCTAATCTTGCTGCACAA-CTCGAG-TTGTGCAGCAAGATTAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTAATCTTGCTGCACAACTCGAGTTGTGCAGCAAGATTAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTAATCTTGCTGCACAACTCGAGTTGTGCAGCAAGATTAGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000301874