Construct: shRNA TRCN0000086319

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011957.1-1294s1c1
DNA Barcode:
CGAAGCCTACTGTTCTACGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Creb3l1 (26427)
Gene Description:
cAMP responsive element binding protein 3-like 1
Transcript:
RefSeq NM_011957.1 (NON-CURRENT)
Match location:
Position 1294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086319)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGCCTACTGTTCTACGAT
Hairpin Sequence:
5'-CCGG-CGAAGCCTACTGTTCTACGAT-CTCGAG-ATCGTAGAACAGTAGGCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGCCTACTGTTCTACGATCTCGAGATCGTAGAACAGTAGGCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGCCTACTGTTCTACGATCTCGAGATCGTAGAACAGTAGGCTTCG-3'

Other clones with same target sequence:

(none)