Construct: shRNA TRCN0000086344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058212.1-756s1c1
DNA Barcode:
CGGGACAGTCATTCCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dpf3 (70127)
Gene Description:
D4, zinc and double PHD fingers, family 3
Transcript:
RefSeq NM_058212.1 (NON-CURRENT)
Match location:
Position 756 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGACAGTCATTCCTAATAA
Hairpin Sequence:
5'-CCGG-CGGGACAGTCATTCCTAATAA-CTCGAG-TTATTAGGAATGACTGTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGACAGTCATTCCTAATAACTCGAGTTATTAGGAATGACTGTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGACAGTCATTCCTAATAACTCGAGTTATTAGGAATGACTGTCCCG-3'

Other clones with same target sequence:

(none)