Construct: shRNA TRCN0000086388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009261.1-2138s1c1
DNA Barcode:
GCTGAGATATAGTGACTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Strbp (20744)
Gene Description:
spermatid perinuclear RNA binding protein
Transcript:
RefSeq NM_009261.1 (NON-CURRENT)
Match location:
Position 2138 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGATATAGTGACTGTAA
Hairpin Sequence:
5'-CCGG-GCTGAGATATAGTGACTGTAA-CTCGAG-TTACAGTCACTATATCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGATATAGTGACTGTAACTCGAGTTACAGTCACTATATCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGATATAGTGACTGTAACTCGAGTTACAGTCACTATATCTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000315988