Construct: shRNA TRCN0000086400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172863.1-312s1c1
DNA Barcode:
ACCCACAGGATGATGATCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp697 (242109)
Gene Description:
zinc finger protein 697
Transcript:
RefSeq NM_172863.1 (NON-CURRENT)
Match location:
Position 312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCACAGGATGATGATCTAA
Hairpin Sequence:
5'-CCGG-ACCCACAGGATGATGATCTAA-CTCGAG-TTAGATCATCATCCTGTGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCACAGGATGATGATCTAACTCGAGTTAGATCATCATCCTGTGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCACAGGATGATGATCTAACTCGAGTTAGATCATCATCCTGTGGGT-3'

Other clones with same target sequence:

(none)