Construct: shRNA TRCN0000086425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019683.2-676s1c1
DNA Barcode:
CCTGATGAATCGTTACATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd49 (56503)
Gene Description:
ankyrin repeat domain 49
Transcript:
RefSeq NM_019683.2 (NON-CURRENT)
Match location:
Position 676 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGAATCGTTACATCAA
Hairpin Sequence:
5'-CCGG-CCTGATGAATCGTTACATCAA-CTCGAG-TTGATGTAACGATTCATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGAATCGTTACATCAACTCGAGTTGATGTAACGATTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGAATCGTTACATCAACTCGAGTTGATGTAACGATTCATCAGG-3'

Other clones with same target sequence:

(none)