Construct: shRNA TRCN0000086467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130895.1-2182s1c1
DNA Barcode:
AGATCACTAAGCCTACCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adarb1 (110532)
Gene Description:
adenosine deaminase, RNA-specific, B1
Transcript:
RefSeq NM_130895.1 (NON-CURRENT)
Match location:
Position 2182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATCACTAAGCCTACCACAT
Hairpin Sequence:
5'-CCGG-AGATCACTAAGCCTACCACAT-CTCGAG-ATGTGGTAGGCTTAGTGATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATCACTAAGCCTACCACATCTCGAGATGTGGTAGGCTTAGTGATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATCACTAAGCCTACCACATCTCGAGATGTGGTAGGCTTAGTGATCT-3'

Other clones with same target sequence:

(none)