Construct: shRNA TRCN0000086473

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019941.1-3002s1c1
DNA Barcode:
CCCTGCAAGTAAGGCTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp235 (56525)
Gene Description:
zinc finger protein 235
Transcript:
RefSeq NM_019941.1 (NON-CURRENT)
Match location:
Position 3002 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086473)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCAAGTAAGGCTCAAAT
Hairpin Sequence:
5'-CCGG-CCCTGCAAGTAAGGCTCAAAT-CTCGAG-ATTTGAGCCTTACTTGCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCAAGTAAGGCTCAAATCTCGAGATTTGAGCCTTACTTGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCAAGTAAGGCTCAAATCTCGAGATTTGAGCCTTACTTGCAGGG-3'

Other clones with same target sequence:

(none)