Construct: shRNA TRCN0000086523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153154.1-1621s1c1
DNA Barcode:
CTGCATCAAACAGAATCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tfap2d (226896)
Gene Description:
transcription factor AP-2, delta
Transcript:
RefSeq NM_153154.1 (NON-CURRENT)
Match location:
Position 1621 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCATCAAACAGAATCTATT
Hairpin Sequence:
5'-CCGG-CTGCATCAAACAGAATCTATT-CTCGAG-AATAGATTCTGTTTGATGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCATCAAACAGAATCTATTCTCGAGAATAGATTCTGTTTGATGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCATCAAACAGAATCTATTCTCGAGAATAGATTCTGTTTGATGCAG-3'

Other clones with same target sequence:

(none)