Construct: shRNA TRCN0000086540

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183289.1-708s1c1
DNA Barcode:
ACTGTGGTATTAGCAGCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcerg1l (70571)
Gene Description:
transcription elongation regulator 1-like
Transcript:
RefSeq NM_183289.1 (NON-CURRENT)
Match location:
Position 708 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086540)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGTGGTATTAGCAGCTCAA
Hairpin Sequence:
5'-CCGG-ACTGTGGTATTAGCAGCTCAA-CTCGAG-TTGAGCTGCTAATACCACAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGTGGTATTAGCAGCTCAACTCGAGTTGAGCTGCTAATACCACAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGTGGTATTAGCAGCTCAACTCGAGTTGAGCTGCTAATACCACAGT-3'

Other clones with same target sequence:

(none)