Construct: shRNA TRCN0000086593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008238.1-2094s1c1
DNA Barcode:
CTCACCTTAAAGGTCAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Foxn1 (15218)
Gene Description:
forkhead box N1
Transcript:
RefSeq NM_008238.1 (NON-CURRENT)
Match location:
Position 2094 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACCTTAAAGGTCAAAGAA
Hairpin Sequence:
5'-CCGG-CTCACCTTAAAGGTCAAAGAA-CTCGAG-TTCTTTGACCTTTAAGGTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACCTTAAAGGTCAAAGAACTCGAGTTCTTTGACCTTTAAGGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACCTTAAAGGTCAAAGAACTCGAGTTCTTTGACCTTTAAGGTGAG-3'

Other clones with same target sequence:

(none)