Construct: shRNA TRCN0000086697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178645.1-161s1c1
DNA Barcode:
GCTTTGATCCAGAAACTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Blmh (104184)
Gene Description:
bleomycin hydrolase
Transcript:
RefSeq NM_178645.1 (NON-CURRENT)
Match location:
Position 161 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGATCCAGAAACTAAAT
Hairpin Sequence:
5'-CCGG-GCTTTGATCCAGAAACTAAAT-CTCGAG-ATTTAGTTTCTGGATCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGATCCAGAAACTAAATCTCGAGATTTAGTTTCTGGATCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGATCCAGAAACTAAATCTCGAGATTTAGTTTCTGGATCAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000327558