Construct: shRNA TRCN0000086773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_133542.4-694s1c1
DNA Barcode:
CGCTGCAAAGTAGTGACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adamts17 (233332)
Gene Description:
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 17
Transcript:
RefSeq XM_133542.4 (NON-CURRENT)
Match location:
Position 694 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGCAAAGTAGTGACAGAA
Hairpin Sequence:
5'-CCGG-CGCTGCAAAGTAGTGACAGAA-CTCGAG-TTCTGTCACTACTTTGCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGCAAAGTAGTGACAGAACTCGAGTTCTGTCACTACTTTGCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGCAAAGTAGTGACAGAACTCGAGTTCTGTCACTACTTTGCAGCG-3'

Other clones with same target sequence:

(none)