Construct: shRNA TRCN0000086781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_487608.1-494s1c1
DNA Barcode:
CTAGTGTTCTTCAGTGTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC435633 (435633)
Gene Description:
similar to RIKEN cDNA 5330426L24
Transcript:
RefSeq XM_487608.1 (NON-CURRENT)
Match location:
Position 494 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGTGTTCTTCAGTGTCTAT
Hairpin Sequence:
5'-CCGG-CTAGTGTTCTTCAGTGTCTAT-CTCGAG-ATAGACACTGAAGAACACTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGTGTTCTTCAGTGTCTATCTCGAGATAGACACTGAAGAACACTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGTGTTCTTCAGTGTCTATCTCGAGATAGACACTGAAGAACACTAG-3'

Other clones with same target sequence:

(none)