Construct: shRNA TRCN0000086790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_283765.2-850s1c1
DNA Barcode:
CCAAACATTCAGGTCACCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ism1 (319909)
Gene Description:
isthmin 1, angiogenesis inhibitor
Transcript:
RefSeq XM_283765.2 (NON-CURRENT)
Match location:
Position 850 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACATTCAGGTCACCATA
Hairpin Sequence:
5'-CCGG-CCAAACATTCAGGTCACCATA-CTCGAG-TATGGTGACCTGAATGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACATTCAGGTCACCATACTCGAGTATGGTGACCTGAATGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACATTCAGGTCACCATACTCGAGTATGGTGACCTGAATGTTTGG-3'

Other clones with same target sequence:

(none)