Construct: shRNA TRCN0000086820

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486010.2-176s1c1
DNA Barcode:
CGAGAACGCTTCCAGGGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prss36 (77613)
Gene Description:
protease, serine 36
Transcript:
RefSeq XM_486010.2 (NON-CURRENT)
Match location:
Position 176 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086820)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAACGCTTCCAGGGACTT
Hairpin Sequence:
5'-CCGG-CGAGAACGCTTCCAGGGACTT-CTCGAG-AAGTCCCTGGAAGCGTTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAACGCTTCCAGGGACTTCTCGAGAAGTCCCTGGAAGCGTTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAACGCTTCCAGGGACTTCTCGAGAAGTCCCTGGAAGCGTTCTCG-3'

Other clones with same target sequence:

(none)