Construct: shRNA TRCN0000086852

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_489163.1-47s1c1
DNA Barcode:
ACCAATACCTTTGTCTTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434126 (434126)
Gene Description:
hypothetical LOC434126
Transcript:
RefSeq XM_489163.1 (NON-CURRENT)
Match location:
Position 47 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086852)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAATACCTTTGTCTTGATA
Hairpin Sequence:
5'-CCGG-ACCAATACCTTTGTCTTGATA-CTCGAG-TATCAAGACAAAGGTATTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAATACCTTTGTCTTGATACTCGAGTATCAAGACAAAGGTATTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAATACCTTTGTCTTGATACTCGAGTATCAAGACAAAGGTATTGGT-3'

Other clones with same target sequence:

(none)