Construct: shRNA TRCN0000086858
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_122700.3-183s1c1
- DNA Barcode:
- CTCTGATTACAACATCCAGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gm1821 (218963)
- Gene Description:
- predicted gene 1821; ubiquitin pseudogene
- Transcript:
- RefSeq XM_122700.3 (NON-CURRENT)
- Match location:
- Position 183 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000086858)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTCTGATTACAACATCCAGAA
- Hairpin Sequence:
-
5'-CCGG-CTCTGATTACAACATCCAGAA-CTCGAG-TTCTGGATGTTGTAATCAGAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTCTGATTACAACATCCAGAACTCGAGTTCTGGATGTTGTAATCAGAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTCTGATTACAACATCCAGAACTCGAGTTCTGGATGTTGTAATCAGAG-3'
Other clones with same target sequence:
(none)
