Construct: shRNA TRCN0000086876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172655.1-320s1c1
DNA Barcode:
CCTCATTATCTTCTGGGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hecw2 (329152)
Gene Description:
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_172655.1 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATTATCTTCTGGGACAT
Hairpin Sequence:
5'-CCGG-CCTCATTATCTTCTGGGACAT-CTCGAG-ATGTCCCAGAAGATAATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATTATCTTCTGGGACATCTCGAGATGTCCCAGAAGATAATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATTATCTTCTGGGACATCTCGAGATGTCCCAGAAGATAATGAGG-3'

Other clones with same target sequence:

(none)