Construct: shRNA TRCN0000086877
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172655.1-482s1c1
- DNA Barcode:
- CTACTTCATGGAACCGGAGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hecw2 (329152)
- Gene Description:
- HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
- Transcript:
- RefSeq NM_172655.1 (NON-CURRENT)
- Match location:
- Position 482 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000086877)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTACTTCATGGAACCGGAGAT
- Hairpin Sequence:
-
5'-CCGG-CTACTTCATGGAACCGGAGAT-CTCGAG-ATCTCCGGTTCCATGAAGTAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTACTTCATGGAACCGGAGATCTCGAGATCTCCGGTTCCATGAAGTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTACTTCATGGAACCGGAGATCTCGAGATCTCCGGTTCCATGAAGTAG-3'
Other clones with same target sequence:
(none)
