Construct: shRNA TRCN0000086931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_488579.1-437s1c1
DNA Barcode:
ACTGTGTGTAGAGGCAGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC432510 (432510)
Gene Description:
hypothetical gene supported by NM_146237
Transcript:
RefSeq XM_488579.1 (NON-CURRENT)
Match location:
Position 437 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGTGTGTAGAGGCAGTCAA
Hairpin Sequence:
5'-CCGG-ACTGTGTGTAGAGGCAGTCAA-CTCGAG-TTGACTGCCTCTACACACAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGTGTGTAGAGGCAGTCAACTCGAGTTGACTGCCTCTACACACAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGTGTGTAGAGGCAGTCAACTCGAGTTGACTGCCTCTACACACAGT-3'

Other clones with same target sequence:

(none)