Construct: shRNA TRCN0000086943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_134498.6-1903s1c1
DNA Barcode:
GCCCTTCATAACTTATCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Neto2 (74513)
Gene Description:
neuropilin (NRP) and tolloid (TLL)-like 2
Transcript:
RefSeq XM_134498.6 (NON-CURRENT)
Match location:
Position 1903 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTCATAACTTATCATAA
Hairpin Sequence:
5'-CCGG-GCCCTTCATAACTTATCATAA-CTCGAG-TTATGATAAGTTATGAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTCATAACTTATCATAACTCGAGTTATGATAAGTTATGAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTCATAACTTATCATAACTCGAGTTATGATAAGTTATGAAGGGC-3'

Other clones with same target sequence:

(none)