Construct: shRNA TRCN0000086958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_489097.2-1034s1c1
DNA Barcode:
CCTGGGATGTATGCTTGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433915 (433915)
Gene Description:
hypothetical gene supported by BC020078; NM_146237
Transcript:
RefSeq XM_489097.2 (NON-CURRENT)
Match location:
Position 1034 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGATGTATGCTTGTTAT
Hairpin Sequence:
5'-CCGG-CCTGGGATGTATGCTTGTTAT-CTCGAG-ATAACAAGCATACATCCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGATGTATGCTTGTTATCTCGAGATAACAAGCATACATCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGATGTATGCTTGTTATCTCGAGATAACAAGCATACATCCCAGG-3'

Other clones with same target sequence:

(none)