Construct: shRNA TRCN0000086979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358650.2-620s1c1
DNA Barcode:
GAATGCTACAAGAGCATACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381751 (381751)
Gene Description:
hypothetical LOC381751
Transcript:
RefSeq XM_358650.2 (NON-CURRENT)
Match location:
Position 620 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000086979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGCTACAAGAGCATACTA
Hairpin Sequence:
5'-CCGG-GAATGCTACAAGAGCATACTA-CTCGAG-TAGTATGCTCTTGTAGCATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGCTACAAGAGCATACTACTCGAGTAGTATGCTCTTGTAGCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGCTACAAGAGCATACTACTCGAGTAGTATGCTCTTGTAGCATTC-3'

Other clones with same target sequence:

(none)