Construct: shRNA TRCN0000087018

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_489649.1-929s1c1
DNA Barcode:
ACCAGTTGGATGGATTTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG386042 (386042)
Gene Description:
predicted gene, EG386042
Transcript:
RefSeq XM_489649.1 (NON-CURRENT)
Match location:
Position 929 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087018)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAGTTGGATGGATTTGATT
Hairpin Sequence:
5'-CCGG-ACCAGTTGGATGGATTTGATT-CTCGAG-AATCAAATCCATCCAACTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAGTTGGATGGATTTGATTCTCGAGAATCAAATCCATCCAACTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAGTTGGATGGATTTGATTCTCGAGAATCAAATCCATCCAACTGGT-3'

Other clones with same target sequence:

(none)