Construct: shRNA TRCN0000087039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_489096.1-828s1c1
DNA Barcode:
AGGACCAGATGGAACCTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433911 (433911)
Gene Description:
hypothetical gene supported by NM_146237
Transcript:
RefSeq XM_489096.1 (NON-CURRENT)
Match location:
Position 828 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACCAGATGGAACCTCTTT
Hairpin Sequence:
5'-CCGG-AGGACCAGATGGAACCTCTTT-CTCGAG-AAAGAGGTTCCATCTGGTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACCAGATGGAACCTCTTTCTCGAGAAAGAGGTTCCATCTGGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACCAGATGGAACCTCTTTCTCGAGAAAGAGGTTCCATCTGGTCCT-3'

Other clones with same target sequence:

(none)