Construct: shRNA TRCN0000087053
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_484217.2-6045s1c1
- DNA Barcode:
- GCCAAGTTTATTTACGTGATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hecw1 (94253)
- Gene Description:
- HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
- Transcript:
- RefSeq XM_484217.2 (NON-CURRENT)
- Match location:
- Position 6045 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000087053)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCAAGTTTATTTACGTGATA
- Hairpin Sequence:
-
5'-CCGG-GCCAAGTTTATTTACGTGATA-CTCGAG-TATCACGTAAATAAACTTGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCAAGTTTATTTACGTGATACTCGAGTATCACGTAAATAAACTTGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCAAGTTTATTTACGTGATACTCGAGTATCACGTAAATAAACTTGGC-3'
Other clones with same target sequence:
(none)
