Construct: shRNA TRCN0000087063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484347.1-1331s1c1
DNA Barcode:
CCAACCTAAGACAGGGATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC432823 (432823)
Gene Description:
similar to hypothetical protein MGC37588
Transcript:
RefSeq XM_484347.1 (NON-CURRENT)
Match location:
Position 1331 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACCTAAGACAGGGATCAA
Hairpin Sequence:
5'-CCGG-CCAACCTAAGACAGGGATCAA-CTCGAG-TTGATCCCTGTCTTAGGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACCTAAGACAGGGATCAACTCGAGTTGATCCCTGTCTTAGGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACCTAAGACAGGGATCAACTCGAGTTGATCCCTGTCTTAGGTTGG-3'

Other clones with same target sequence:

(none)