Construct: shRNA TRCN0000087081

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486461.1-113s1c1
DNA Barcode:
TCCATGTGAATGCAACTGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434613 (434613)
Gene Description:
similar to murinoglubulin 4 precursor
Transcript:
RefSeq XM_486461.1 (NON-CURRENT)
Match location:
Position 113 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087081)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCATGTGAATGCAACTGTTA
Hairpin Sequence:
5'-CCGG-TCCATGTGAATGCAACTGTTA-CTCGAG-TAACAGTTGCATTCACATGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCATGTGAATGCAACTGTTACTCGAGTAACAGTTGCATTCACATGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCATGTGAATGCAACTGTTACTCGAGTAACAGTTGCATTCACATGGA-3'

Other clones with same target sequence:

(none)