Construct: shRNA TRCN0000087121

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485068.1-144s1c1
DNA Barcode:
CGAGCTATGTTTAGAGTGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433477 (433477)
Gene Description:
similar to proteasome 26S non-ATPase subunit 11
Transcript:
RefSeq XM_485068.1 (NON-CURRENT)
Match location:
Position 144 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087121)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCTATGTTTAGAGTGCAT
Hairpin Sequence:
5'-CCGG-CGAGCTATGTTTAGAGTGCAT-CTCGAG-ATGCACTCTAAACATAGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCTATGTTTAGAGTGCATCTCGAGATGCACTCTAAACATAGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCTATGTTTAGAGTGCATCTCGAGATGCACTCTAAACATAGCTCG-3'

Other clones with same target sequence:

(none)