Construct: shRNA TRCN0000087135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484760.1-271s1c1
DNA Barcode:
GCATGTTACTTCTGCCTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433213 (433213)
Gene Description:
hypothetical LOC433213
Transcript:
RefSeq XM_484760.1 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTTACTTCTGCCTAGAA
Hairpin Sequence:
5'-CCGG-GCATGTTACTTCTGCCTAGAA-CTCGAG-TTCTAGGCAGAAGTAACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTTACTTCTGCCTAGAACTCGAGTTCTAGGCAGAAGTAACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTTACTTCTGCCTAGAACTCGAGTTCTAGGCAGAAGTAACATGC-3'

Other clones with same target sequence:

(none)