Construct: shRNA TRCN0000087148
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_148584.5-4285s1c1
- DNA Barcode:
- GCGTAGCTTTATTTGAGTCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Usp7 (252870)
- Gene Description:
- ubiquitin specific peptidase 7
- Transcript:
- RefSeq XM_148584.5 (NON-CURRENT)
- Match location:
- Position 4285 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000087148)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGTAGCTTTATTTGAGTCTT
- Hairpin Sequence:
-
5'-CCGG-GCGTAGCTTTATTTGAGTCTT-CTCGAG-AAGACTCAAATAAAGCTACGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGTAGCTTTATTTGAGTCTTCTCGAGAAGACTCAAATAAAGCTACGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGTAGCTTTATTTGAGTCTTCTCGAGAAGACTCAAATAAAGCTACGC-3'
Other clones with same target sequence:
-
TRCN0000349544
