Construct: shRNA TRCN0000087150

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_148584.5-1921s1c1
DNA Barcode:
CCTGCAATGTTAGATAATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp7 (252870)
Gene Description:
ubiquitin specific peptidase 7
Transcript:
RefSeq XM_148584.5 (NON-CURRENT)
Match location:
Position 1921 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087150)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCAATGTTAGATAATGAA
Hairpin Sequence:
5'-CCGG-CCTGCAATGTTAGATAATGAA-CTCGAG-TTCATTATCTAACATTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCAATGTTAGATAATGAACTCGAGTTCATTATCTAACATTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCAATGTTAGATAATGAACTCGAGTTCATTATCTAACATTGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000318333