Construct: shRNA TRCN0000087155

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356875.3-240s1c1
DNA Barcode:
CCCAGTGCTCACTCAGGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm1246 (383101)
Gene Description:
gene model 1246, (NCBI)
Transcript:
RefSeq XM_356875.3 (NON-CURRENT)
Match location:
Position 240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087155)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTGCTCACTCAGGTCAA
Hairpin Sequence:
5'-CCGG-CCCAGTGCTCACTCAGGTCAA-CTCGAG-TTGACCTGAGTGAGCACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTGCTCACTCAGGTCAACTCGAGTTGACCTGAGTGAGCACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTGCTCACTCAGGTCAACTCGAGTTGACCTGAGTGAGCACTGGG-3'

Other clones with same target sequence:

(none)