Construct: shRNA TRCN0000087165

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484726.1-281s1c1
DNA Barcode:
TCTGCCTAGAACAATTAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spink11 (433181)
Gene Description:
serine peptidase inhibitor, Kazal type 11
Transcript:
RefSeq XM_484726.1 (NON-CURRENT)
Match location:
Position 281 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000087165)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGCCTAGAACAATTAGTAT
Hairpin Sequence:
5'-CCGG-TCTGCCTAGAACAATTAGTAT-CTCGAG-ATACTAATTGTTCTAGGCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGCCTAGAACAATTAGTATCTCGAGATACTAATTGTTCTAGGCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGCCTAGAACAATTAGTATCTCGAGATACTAATTGTTCTAGGCAGA-3'

Other clones with same target sequence:

(none)